The Raman microprobe is a popular accessory compatible with both the HoloLab™ and RamanRxn Systems™ product lines. The Raman microprobe provides complementary microspectroscopy information on fundamental molecular vibrations to mid-infrared FT-IR microscopes. Using excitation wavelengths in the visible spectral region, a Raman microprobe is capable of analyzing diffraction-limited areas (typically 1 micron). The ability to both identify areas 10 times smaller in size than can be determined by FT-IR microscopy and the non-contact / no sample preparation nature of the technique are some of the many advantages of Raman microscopy.

The Raman microprobe can be configured with optional high-precision mapping stages and confocal optics to realize both surface and volumetric chemical imaging.
The microprobe can be offered with an optional laser radiation light shield for operation in a standard laboratory environment.

The combination of the fiber-coupled spectrometer and microscope in conjunction with a thermally stabilized microscope and high-precision motorized XYZ stage allows for routine collection of Raman images.

Our Windows® 2000–compatible HoloGRAMS™ software and HoloMap™ software make real-time chemical images from Raman spectroscopy truly feasible.

Pharmaceutical slab image showing Raman images of the active material, a filler and an impurity overlaid onto a white light reflectance image of the sample

Features

• Patented thermally stabilized optical microscope
   
• Transmission illumination
   
• Reflected light illumination
   
• Real-time color CCD camera with PC-compatible image grabbing system
   
• Real-time continuous viewing of laser spot in white light and Raman mode
   
• Autofocus mode
   

Software

• HoloGRAMS 4.0 software package
   
• HoloMap software package
   
  

Optional Features

• Light shield
   
• Class I light shield
   
• Polarization light upgrade
   
• Motorized stage/mapping
   
• Long working distance objectives

Microprobe

• Complementary spectroscopic information to FT-IR microscopy
   
• High spatial resolution (1 micron) with true confocal design for depth discrimination (2 microns)
   
• Simultaneous full spectral coverage provides survey information at all sample positions
   
• Integrated real-time color video display and capture with direct viewing of the laser location
   
• Microscope enclosure and mapping stage options
   
• Autofocus mode for rough samples allows for maximum signal collection during long acquisitions and identification of surface profile effects

HoloGRAMS/HoloMap Mapping Software

• High-precision XYZ mapping stages
   
• Interactive display and setup featuring direct viewing of both laser position and all mapping positions
   
• Point, line, area, and volumetric data acquisition modes plus multiple imaging modes for extended operation
   
• Interlinked 2D and 3D chemical image extraction modes
   
• Optical and chemical image overlays

FastMap™ Chemical Imaging Software

• Confocal Raman imaging
   
• Full spectral data recorded in a fraction of the time required for conventional point-map Raman microscopy using a combination of the HoloPlex™ advantage and FastMap software
   
• Quantitative chemical distribution information available
   
• Data extraction and manipulation tools available for chemical image enhancement
   
• Surface topology information