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Validating Transcutaneous Raman Spectroscopy in Human Subjects
Raman spectroscopy can non-destructively measure properties of bone related to mineral density, mineral composition, and collagen composition. Bone properties can be measured through the skin in animal and human subjects, but correlations between transcutaneous and exposed bone measurements in humans have previously only been reported for cadavers. In an ongoing study we are collecting in vivo transcutaneous Raman measurements, exposed bone measurements collected in vivo during surgeries, and ex vivo exposed bone Raman measurements collected on bone fragments recovered during surgery. This data will be used to demonstrate in vivo feasibility and to compare transcutaneous and exposed Raman spectroscopy of bone.
Challenges specific to in vivo biomedical measurements will be discussed for both clinical and surgical applications. To simplify further medical translation of our transcutaneous measurements, commercial Raman instrumentation (RamanRxn1 spectrograph and PhAT optical probe) operating at 785 nm excitation are used for these in vivo measurements. Instrument customizations specific to the in vivo work will be described, along with the customized control software and data processing steps which enable enhanced spectral recovery.
AIR DATE: April 11, 2013
Name: Dr. Francis Esmonde-White
Title: Research Area Specialist Lead, Dept of Chemistry
Company: University of Michigan